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Nanoparticle displacement assay with electrochemical nanopore-based sensors

Lautner, Gergely and Plesz, Mónika and Jágerszki, Gyula and Fürjes, Péter and Gyurcsányi, Ervin Róbert (2016) Nanoparticle displacement assay with electrochemical nanopore-based sensors. ELECTROCHEMISTRY COMMUNICATIONS, 71. pp. 13-17. ISSN 1388-2481

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Abstract

The proof of concept of a nanoparticle displacement assay that enables the use of large diameter nanopores for the detection of targets of smaller molecular dimensions is presented. We hypothesized that an inherent signal amplification should arise from the selective displacement of nanoparticles preloaded in a nanopore by a much smaller molecular target. The method is demonstrated using peptide nucleic acid (PNA)-functionalized gold nanopore arrays in which short DNA-modified gold nanoparticles are anchored by weak interaction. Complementary microRNAs are detected via the resistance change caused by competitive displacement of nanoparticles from the PNA-functionalized nanopores. © 2016 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: RNA; Nucleic Acids; NANOPARTICLES; nanoparticle; microRNA; GOLD; MICRORNAS; GOLD NANOPARTICLES; Nanopores; PNA; peptide nucleic acid; Displacement assay; SOLID-STATE NANOPORE; Signal amplifications; NASBA; Molecular dimensions; Solid state nanopore;
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD02 Physical chemistry / fizikai kémia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 30 Nov 2020 12:18
Last Modified: 30 Nov 2020 12:18
URI: http://real.mtak.hu/id/eprint/117568

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