Soós, Vilmos and Shetty, Prateek and Maróti, Gergely and Incze, Norbert and Badics, Eszter and Balázs, Ervin (2021) Biomolecule composition and draft genome of a novel, high-lipid producing Scenedesmaceae microalga. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 54. ISSN 2211-9264
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Abstract
Lipid biosynthesis in microalgae can be stimulated by cultivation in low nitrogen medium. MACC-401 was isolated from the soil surface in Tres Marias (MG-Brazil). The strain shows the morphological characteristics of the Scenedesmaceae green algae. The daily biomass and lipid production of MACC-401 is remarkable, 0.36 g L−1 and 110 mg L−1, respectively. Exploration of the genetic background of this promising strain not only allows the utilization of this species for industrial-scale lipid production, but also provides genetic targets to select lipid-producing strains from microalgae collections. We conducted physiological experiments by cultivating MACC-401 in complete and N-limited media and performed genome sequencing as well as transcriptome analysis. The estimated nuclear genome size of MACC-401 is 99.503 Mbp and the chloroplast genome is 0.15 Mbp. The phylogenetic analysis confirmed that the MACC-401 belongs to the Scenedesmaceae family, and represents a genetically distinct accession in this family. A basic comparative transcriptome analysis resulted in the identification of N-starvation responsive genes, which could serve as markers to monitor the onset of lipid accumulation in algal cultures.
Item Type: | Article |
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Uncontrolled Keywords: | Transcriptome; genome sequencing; lipid accumulation; Scenedesmaceae; N-starvation; |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia Q Science / természettudomány > QK Botany / növénytan |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 02 Sep 2021 05:19 |
Last Modified: | 02 Sep 2021 05:19 |
URI: | http://real.mtak.hu/id/eprint/128965 |
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