Pomothy, Judit and Gatt, Katrina and Jerzsele, Ákos and Pásztiné Gere, Erzsébet (2020) The impact of quercetin on a porcine intestinal epithelial cell line exposed to deoxynivalenol. ACTA VETERINARIA HUNGARICA, 68 (4). pp. 380-386. ISSN 0236-6290
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Abstract
Quercetin (Que) is present in many vegetables and fruits as a secondary antioxidant metabolite. Deoxynivalenol (DON) produced by various Fusarium mould species can induce cytotoxicity and oxidative stress in the gastrointestinal tracts of humans and farm animals. The aim of this study was to investigate the effects of Que on DON-induced oxidative stress in a non-tumourigenic porcine IPEC-J2 cell line. Two experimental designs were used in our experiments as follows: (a) pretreatment with 20 mu mol/L Que for 24 h followed by 1-h 1 mu mol/L DON treatment and (b) simultaneous application of 20 mu mol/L Que and 1 mu mol/L DON for 1 h. Cell cytotoxicity, transepithelial electrical resistance (TER) of cell monolayers and extracellular/intracellular redox status were studied. It was found that DON significantly decreased TER and triggered oxidative stress, while Que pretreatments were beneficial in maintaining the integrity of the monolayers and alleviated oxidative stress. However, co-treatment with Que was unable to preserve the integrity and redox balance of the cells exposed to DON. These results indicate that only the 24-h preincubation of cells with 20 mu mol/L Que was beneficial in compensating for the disruption caused by DON in extracellular oxidative status.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [115685, 124522]; European UnionEuropean Commission; European Social FundEuropean Social Fund (ESF) [EFOP-3.6.1-16-2016-00024, EFOP-3.6.2-16-2017-00012, EFOP-3.6.3-VEKOP-16-2017-00005]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences Funding text: This research was supported by the Hungarian Scientific Research Fund (grant numbers 115685 and 124522). The work was supported by the European Union and cofinanced by the European Social Fund (grant agreement no. EFOP-3.6.1-16-2016-00024, EFOP-3.6.2-16-2017-00012 and EFOP-3.6.3-VEKOP-16-2017-00005). The project received support from the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. We would like to express special thanks to Julia Seprodi for the chemical background and to Agnes Eszter Czimmermann and Reka Fanni Barna for their technical support. |
| Subjects: | S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 08 Sep 2021 05:08 |
| Last Modified: | 08 Sep 2021 05:08 |
| URI: | http://real.mtak.hu/id/eprint/129133 |
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