Abbasi, Elham and Goudarzi, Hossein and Hashemi, Ali and Chirani, Alireza Salimi and Ardebili, Abdollah (2021) Decreased carO gene expression and OXA-type carbapenemases among extensively drug-resistant Acinetobacter baumannii strains isolated from burn patients in Tehran, Iran. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 68 (1). pp. 48-54. ISSN 1217-8950
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Abstract
A major challenge in the treatment of infections has been the rise of extensively drug resistance (XDR) and multidrug resistance (MDR) in Acinetobacter baumannii. The goals of this study were to determine the pattern of antimicrobial susceptibility, bla(OXA) and carO genes among burn-isolated A. baumannii strains. In this study, 100 A. baumannii strains were isolated from burn patients and their susceptibilities to different antibiotics were determined using disc diffusion testing and broth microdilution. Presence of carO gene and OXA-type carbapenemase genes was tested by PCR and sequencing. SDS-PAGE was done to survey CarO porin and the expression level of carO gene was evaluated by Real-Time PCR. A high rate of resistance to meropenem (98%), imipenem (98%) and doripenem (98%) was detected. All tested A. baumannii strains were susceptible to colistin. The results indicated that 84.9% were XDR and 97.9% of strains were MDR. In addition, all strains bore bla(OXA-51) like and bla(OXA-23) like and carO genes. Nonetheless, bla(OXA-58) like and bla(OXA-24) like genes were harbored by 0 percent and 76 percent of strains, respectively. The relative expression levels of the carO gene ranged from 0.06 to 35.01 fold lower than that of carbapenem-susceptible A. baumannii ATCC19606 and SDS - PAGE analysis of the outer membrane protein showed that all 100 isolates produced CarO. The results of current study revealed prevalence of bla(OXA) genes and changes in carO gene expression in carbapenem resistant A.baumannii.
| Item Type: | Article |
|---|---|
| Additional Information: | Funding Agency and Grant Number: research Department of the School of Medicine, Shahid Beheshti University of Medical Sciences [9891] |
| Uncontrolled Keywords: | MECHANISMS; DIVERSITY; immunology; emergence; imipenem; Molecular Epidemiology; efflux pump; Multi-drug resistance; Acinetobacter baumannii; carbapenemases; OUTER-MEMBRANE PROTEIN; carO; bla(OXA); |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 15 Oct 2021 07:00 |
| Last Modified: | 31 Mar 2022 23:15 |
| URI: | http://real.mtak.hu/id/eprint/132539 |
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