Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization

Majer, Petra and Czégény, Gyula and Sándor, Györgyi and Dix, Phil J. and Hideg, Éva (2014) Antioxidant defence in UV-irradiated tobacco leaves is centred on hydrogen-peroxide neutralization. Plant Physiology and Biochemistry, 82. pp. 239-43. ISSN 0981-9428

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Abstract

Greenhouse grown tobacco (Nicotiana tabacum L. cv. Petit Havana) plants were exposed to supplemental UV centred at 318 nm and corresponding to 13.6 kJ m-2 d-1 biologically effective UV-B (280-315 nm) radiation. After 6 days this treatment decreased photosynthesis by 30%. Leaves responded by a large increase in UV-absorbing pigment content and antioxidant capacities. UV-stimulated defence against ROS was strongest in chloroplasts, since activities of plastid enzymes FeSOD and APX had larger relative increases than other, non-plastid specific SODs or peroxidases. In addition, non-enzymatic defence against hydroxyl radicals was doubled in UV treated leaves as compared to controls. In UV treated leaves, the extent of activation of ROS neutralizing capacities followed a peroxidases > hydroxyl-radical neutralization > SOD order. These results suggest that highly effective hydrogen peroxide neutralization is the focal point of surviving UV-inducible oxidative stress and argue against a direct signalling role of hydrogen peroxide in maintaining adaptation to UV, at least in laboratory experiments.

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