Dlouhý, Ondřej and Javornik, Uroš and Zsíros, Ottó and Sket, Primoz and Karlický, Václav and Garab, Győző (2021) Lipid Polymorphism of the Subchloroplast-Granum and Stroma Thylakoid Membrane-Particles. I. P-31-NMR Spectroscopy. CELLS, 10 (9). ISSN 2073-4409
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Abstract
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been documented that fully functional thylakoid membranes, in addition to the bilayer, contain an inverted hexagonal (H-II) phase and two isotropic phases. To shed light on the origin of these non-lamellar phases, we performed P-31-NMR spectroscopy experiments on sub-chloroplast particles of spinach: stacked, granum and unstacked, stroma thylakoid membranes. These membranes exhibited similar lipid polymorphism as the whole thylakoids. Saturation transfer experiments, applying saturating pulses at characteristic frequencies at 5 degrees C, provided evidence for distinct lipid phases-with component spectra very similar to those derived from mathematical deconvolution of the P-31-NMR spectra. Wheat-germ lipase treatment of samples selectively eliminated the phases exhibiting sharp isotropic peaks, suggesting easier accessibility of these lipids compared to the bilayer and the H-II phases. Gradually increasing lipid exchanges were observed between the bilayer and the two isotropic phases upon gradually elevating the temperature from 5 to 35 degrees C, suggesting close connections between these lipid phases. Data concerning the identity and structural and functional roles of different lipid phases will be presented in the accompanying paper.
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| Additional Information: | Funding Agency and Grant Number: Czech Science FoundationGrant Agency of the Czech Republic [GACR 19-13637S]; National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 128679]; project "SustES-Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions" [CZ.02.1.01/0.0/0.0/16_019/0000797]; Silesian Region [07359/2019/RRC]; University of Ostrava [SGS02/PrF/2021] Funding text: This work was supported by grants from the Czech Science Foundation (GAC R 19-13637S to G.G., V.S., V.K. and O.D.), the National Research Development and Innovation Office of Hungary (OTKA K 128679 to G.G.), the project "SustES-Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions" (CZ.02.1.01/0.0/0.0/16_019/0000797, to V.S. and V.K.), the Silesian Region (07359/2019/RRC to O.D.) and the University of Ostrava (SGS02/PrF/2021 to O.D.). The 31P-NMR measurements were carried out in the NMR Center of CERIC-ERIC; this organization also provided financial assistance in shipping samples from Szeged to Ljubljana. |
| Uncontrolled Keywords: | THYLAKOID MEMBRANES; Isotropic phase; bilayer membrane; Grana; H-II phase; P-31-NMR; Non-lamellar lipid phases; DEM-dynamic exchange model; non-bilayer lipids; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia R Medicine / orvostudomány > RC Internal medicine / belgyógyászat |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 03 Feb 2022 12:50 |
| Last Modified: | 03 Feb 2022 12:50 |
| URI: | http://real.mtak.hu/id/eprint/137218 |
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