The DREAM complex represses growth in response to DNA damage in Arabidopsis

Lang, L. and Pettkó-Szandtner, Aladár and Elbasi, H.T. and Takatsuka, H. and Nomoto, Y. and Magyar, Zoltán (2021) The DREAM complex represses growth in response to DNA damage in Arabidopsis. LIFE SCIENCE ALLIANCE, 4 (12). ISSN 2575-1077


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The DNA of all organisms is constantly damaged by physiological processes and environmental conditions. Upon persistent damage, plant growth and cell proliferation are reduced. Based on previous findings that RBR1, the only Arabidopsis homolog of the mammalian tumor suppressor gene retinoblastoma, plays a key role in the DNA damage response in plants, we unravel here the network of RBR1 interactors under DNA stress conditions. This led to the identification of homologs of every DREAM component in Arabidopsis, including previously not recognized homologs of LIN52. Interestingly, we also discovered NAC044, a mediator of DNA damage response in plants and close homolog of the major DNA damage regulator SOG1, to directly interact with RBR1 and the DREAM component LIN37B. Consistently, not only mutants in NAC044 but also the double mutant of the two LIN37 homologs and mutants for the DREAM component E2FB showed reduced sensitivities to DNA-damaging conditions. Our work indicates the existence of multiple DREAM complexes that work in conjunction with NAC044 to mediate growth arrest after DNA damage. © 2021 Rockefeller University Press. All rights reserved.

Item Type: Article
Additional Information: Department of Developmental Biology, University of Hamburg, Institute for Plant Sciences and Microbiology, Hamburg, Germany Laboratory of Proteomic Research, Biological Research Centre, Szeged, Hungary Institute of Plant Biology, Biological Research Centre, Szeged, Hungary Department of Biological Sciences, Centre for Systems and Synthetic Biology, Royal Holloway University of London, Egham, United Kingdom Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium Vlaams Instituut voor Biotechnologie (VIB), Center for Plant Systems Biology, Ghent, Belgium School of Biological Science and Technology, College of Science and Engineering, Kanazawa University, Kanazawa, Japan School of Life Sciences, University of Warwick, Coventry, United Kingdom Export Date: 3 November 2021 Correspondence Address: Heese, M.; Department of Developmental Biology, Germany; email: Correspondence Address: Schnittger, A.; Department of Developmental Biology, Germany; email: Funding details: BB/M025047/1 Funding details: GINOP-2.3.2-15-2016-00032 Funding details: 739593 Funding details: NKFIH-K132486 Funding details: Deutsche Forschungsgemeinschaft, DFG, SCHN 736/16-1 Funding details: Japan Society for the Promotion of Science, KAKEN, 18H04833, 20H05408 Funding details: Universität Hamburg, UH Funding text 1: We thank the Single Cell Omics Advanced Core Facility staff of the Hungarian Centre of Excellence for Molecular Medicine (HCEMM) and Biological Research Center for help with their resources and their support. HCEMM has received funding from the EU’s Horizon 2020 research and innovation program (739593). This work was supported through a fellowship of the University of Hamburg to L Lang, a grant by the Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00032) to A Pettkó-Szandtner, a grant from the Japan Society for the Promotion of Science KAKENHI (20H05408 and 18H04833) to M Ito, a grant from the Hungarian National Research Funding (NKFIH-K132486) to Z Magyar, a BBSRC-NSF grant (BB/M025047/1) to L Bögre, and a DFG grant (SCHN 736/16-1) to A Schnittger.
Subjects: Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan
Depositing User: MTMT SWORD
Date Deposited: 07 Feb 2022 08:45
Last Modified: 07 Feb 2022 08:45

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