Slaska-Kiss, Krystyna and Zsibrita, Nikolett and Koncz, Mihály and Albert, Pál and Csábrádi, Ákos and Szentes, Sarolta and Kiss, Antal (2021) Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli. SCIENTIFIC REPORTS, 11 (1). ISSN 2045-2322
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s41598-021-94528-3.pdf Available under License Creative Commons Attribution. Download (2MB) | Preview |
Official URL: http://doi.org/10.1038/s41598-021-94528-3
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Hungarian Scientific Research Fund (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K83983]; Ministry of Finance of Hungary [GINOP-2.3.2-15-2016-00001]; European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant [642691] Funding text: We thank Bill Jack for the original plasmid containing a modified M.SssI gene, Marianne G. Rots for the plasmids encoding the 6ZB and 6ZA zinc finger proteins and for suggestions on the manuscript. The plasmids pdCas9 (Addgene plasmid #46569; http://n2t.net/addgene:46569; RRID:Addgene_46569) and pCRISPR (Addgene plasmid #42875; http://n2t.net/addgene:42875; RRID:Addgene_42875)-were kind gifts from Luciano Marraffini. We thank Ibolya Anton for the technical assistance, Ildiko Stier for help in an early phase of this work and Akos Nyerges for discussions. This work was supported by the K83983 grant from the Hungarian Scientific Research Fund (OTKA), by the Ministry of Finance of Hungary [Grant No. GINOP-2.3.2-15-2016-00001] and by the European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant Agreement No. 642691. |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 07 Feb 2022 15:14 |
| Last Modified: | 07 Feb 2022 15:14 |
| URI: | http://real.mtak.hu/id/eprint/137528 |
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