Viral Gene Expression Profile of Goose Haemorrhagic Polyomavirus in Susceptible Primary Cells

Kaszab, Eszter and Szabadi, Luca and Kepner, Anett and Bajnóczi, Pál and Lengyel, György and Bányai, Krisztián and Fehér, Enikő (2021) Viral Gene Expression Profile of Goose Haemorrhagic Polyomavirus in Susceptible Primary Cells. AVIAN PATHOLOGY, 50 (5). pp. 447-452. ISSN 0307-9457 (print); 1465-3338 (online)

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Abstract

Routine culturing of goose haemorrhagic polyomavirus (GHPV) is cumbersome, and limited data are available about its replication and gene expression profile. In this study, goose embryo fibroblast cells were infected with GHPV for temporal measurement of the viral genome copy number and mRNA levels with quantitative PCR. Accumulation of small and large tumour antigen-encoding mRNAs was detected as early as 9 hours post-infection (hpi), while high level expression of the capsid protein encoding VP1-VP3, and ORF-X mRNAs was first detected at 24 hpi. Elevation of GHPV genome copy number was noted at 48 hpi. The results indicate that the gene expression profile of GHPV is similar to that described for mammalian polyomaviruses.

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