Presence of immunoreactive endothelin-1 and atrial natriuretic peptide in human pericardial fluid.

Horkay, Ferenc and Szokodi, István and Selmeci, L. and Merkely, Béla Péter and Kékesi, Violetta and Tóth, Miklós (1998) Presence of immunoreactive endothelin-1 and atrial natriuretic peptide in human pericardial fluid. LIFE SCIENCES, 62 (3). pp. 267-274. ISSN 0024-3205

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Abstract

This study was undertaken to characterize endothelin-1 (ET-1) and atrial natriuretic peptide (ANP) concentrations in human pericardial fluid, blood plasma, right atrial appendage and papillary muscle by use of specific radioimmunoassays. In patients undergoing cardiac surgery (n=16) pericardial fluid mean immunoreactive (ir-) ET-1 and ir-ANP levels were 36-fold and 4-fold higher than corresponding plasma levels, respectively. In high performance liquid chromatography (HPLC) pericardial fluid ir-ET-1 was indistinguishable from human ET-1[1-21] and the majority of pericardial fluid ir-ANP coeluted with human ANP[99-126]. Atrial tissue ir-ET-1 and ir-ANP concentrations were 17-fold and 870-fold higher than in ventricular tissue. Our present study demonstrated for the first time the presence of ir-ET-1 in the pericardial fluid in humans. Human pericardial fluid contained far the highest concentrations of ET-1 among all biological fluids tested thus far. The functions of pericardial fluid ET-1 and ANP on cardiac performance and coronary vascular tone require further investigations.

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