A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers

Berényi, Szilvia and Mihály, Judith and Wacha, András Ferenc and Tőke, Orsolya and Bóta, Attila (2014) A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers. COLLOIDS AND SURFACES B-BIOINTERFACES, 118 (1). pp. 164-171. ISSN 0927-7765

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The effect of 5th generation polyamidoamine (PAMAM G5) dendrimers on multilamellar dipalmitoylphosphocholine (DPPC) vesicles was investigated. PAMAM was added in two different concentration to the lipids (10-3 and 10-2 dendrimer/lipid molar ratios). The thermal behavior of the evolved systems was characterized by DSC; while the structure and the morphology were investigated with small- and wide-angel X-ray scattering (SWAXS), freeze-fracture electron microscopy (FFTEM) and phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy, respectively. IR spectroscopy was used to study the molecular interactions between PAMAM and DPPC. The obtained results show that the dendrimers added in 10-3 molar ratio to the lipids generate minor perturbations in the multilamellar structure and thermal character of liposomes, while added in 10-2 molar ratio dendrimers cause major disturbance in the vesicular system. The terminal amino groups of the dendrimers are in strong interaction with the phosphate headgroups and through this binding dendrimers disrupt the regular multilamellar structure of DPPC. Besides highly swollen, fragmented bilayers, small vesicles are formed.

Item Type: Article
Subjects: G Geography. Anthropology. Recreation / földrajz, antropológia, kikapcsolódás > GE Environmental Sciences / környezettudomány
Q Science / természettudomány > QD Chemistry / kémia
Depositing User: MTMT SWORD
Date Deposited: 26 Aug 2014 12:03
Last Modified: 26 Aug 2014 12:03

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