Influence of poly(ethylene oxide)-based copolymer on protein adsorption and bacterial adhesion on stainless steel : Modulation by surface hydrophobicity

Yang, Yi and Rouxhet, Paul G. and Chudziak, Dorota and Telegdi, Lászlóné and Dupont-Gillain, Christine C. (2014) Influence of poly(ethylene oxide)-based copolymer on protein adsorption and bacterial adhesion on stainless steel : Modulation by surface hydrophobicity. BIOELECTROCHEMISTRY, 97. pp. 127-136. ISSN 1567-5394

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Abstract

The aim of the present work is to study the adhesion of Pseudomonas NUMB 2021, a typical aerobic marine microorganism, on stainless steel (SS) substrate. More particularly, the potential effect on adhesion of adsorbed poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer is investigated. Bacterial attachment experiments were carried out using a modified parallel plate flow chamber, allowing different surface treatments to be compared in a single experiment The amount of adhering bacteria was determined via DAPI staining and fluorescence microscopy. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface chemical composition of SS and hydrophobized SS before and after PEO-PPO-PEO adsorption. The adsorption of bovine serum albumin (BSA), a model protein, was investigated to test the resistance of PEO-PPO-PEO layers to protein adsorption. The results show that BSA adsorption and Pseudomonas 2021 adhesion are significantly reduced on hydrophobized SS conditioned with PEO-PPO-PEO. Although PEO-PPO-PEO is also found to adsorb on SS, it does not prevent BSA adsorption nor bacterial adhesion, which is attributed to different PEO-PPO-PEO adlayer structures on hydrophobic and hydrophilic surfaces. The obtained results open the way to a new strategy to reduce biofouling on metal oxide surfaces using PEO-PPO-PEO triblock copolymer. (C) 2013 Elsevier B.V. All rights reserved.

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