Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions

Ádám, Attila and Kátay, György and Künstler, András and Király, Lóránt (2022) Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions. In: Reactive Oxygen Species in Plants. Springer US, New York (NY), pp. 191-200. ISBN 9781071624692; 9781071624685

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Abstract

Azelaic acid (AzA, 1,9-nonadienoic acid) is a nine-carbon chain (C9 ) dicarboxylic acid with multiple and diverse functions in humans and plants. In plants this compound was suggested as a marker for lipid peroxidation under biotic and abiotic stress conditions and an inducer (priming agent) of plant immunity (acquired resistance). Detection methods for AzA in plants include a wide range of methodological approaches. This new and simple reversed-phase HPLC-MS protocol describes the measurement of AzA and other dicarboxylic acids either from tobacco leaf tissue or petiolar exudates (vascular sap) of plants under non-derivatized conditions.

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