Davoudabadi, Sara and Goudarzi, Hossein and Goudarzi, Mehdi and Ardebili, Abdollah and Faghihloo, Ebrahim and Sharahi, Javad Yasbolaghi and Hashemi, Ali (2022) Detection of extensively drug-resistant and hypervirulent Klebsiella pneumoniae ST15, ST147, ST377 and ST442 in Iran. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 69 (1). pp. 77-86. ISSN 1217-8950
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Abstract
In this study, we focused on the emergence of extensively drug-resistant (XDR), pandrug-resistant (PDR), and hypervirulent Klebsiella pneumoniae (hvKP) in Iran. During 2018 to 2020 a total of 52 K. pneumoniae isolates were collected from different clinical specimens. The hvKP isolates were identified by PCR amplification of virulence and capsular serotype-specific genes. Hypermucoviscous K. pneumoniae (hmKP) were identified by string test. Carbapenem-resistant hvKP (CR-hvKP), multidrug-resistant hvKP (MDR-hvKP), extensively drug-resistant hvKP (XDR-hvKP), and pandrug-resistant hvKP (PDR-hvKP) were determined by disc diffusion method, Carba-NP test and PCR method. XDR-hvKP isolates were typed by multilocus sequence typing (MLST). Among all K. pneumoniae isolates 14 (26.9%) were identified as hvKP and 78.6% (11/14) of them were hmKP however, none of the classic K. pneumoniae (cKP) isolates were hmKP. The predominant capsular serotype of hvKP was K2 (42.85%) followed by K1 (35.71%). The prevalence of MDR-hvKP, XDR-hvKP and PDR-hvKP isolates were 6 (42.9%), 5 (35.7%) and 1 (7.1%), respectively. ESBL production was found in 85.7% of hvKP isolates and most of them carried bla(TEM) gene (78.6%) and 6 isolates (42.9%) were CR-hvKP. Among hvKP isolates, 1 (7.1%), 2 (14.3%), 3 (21.4%), 8 (28.6%), and 11 (78.6%) carried bla(NDM-6), bla(OXA-48), bla(CTX-M), bla(SHV), and bla(TEM) genes, respectively. According to MLST analysis, 2, 1, 1, and 1 XDR-hvKP isolates belonged to ST15, ST377, ST442, and ST147, respectively. The occurrence of such isolates is deeply concerning due to the combination of hypervirulence and extensively drug-resistance or pandrug-resistance.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: research Department of the School of Medicine, Shahid Beheshti University of Medical Sciences [20277] Funding text: The present study was financially supported by the research Department of the School of Medicine, Shahid Beheshti University of Medical Sciences (Grant No.: 20277) . |
| Uncontrolled Keywords: | GENES; immunology; plasmid; Virulence Factors; MULTIPLEX PCR; RAPID DETECTION; serotype K1; hypervirulent Klebsiella pneumoniae; Microbiological characteristics; LIVER-ABSCESS; extensively drug-resistance; multidrug-resistant hvKP; pandrug-resistance; COMMUNITY-ACQUIRED BACTEREMIA; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 25 Jul 2022 06:10 |
| Last Modified: | 25 Jul 2022 08:01 |
| URI: | http://real.mtak.hu/id/eprint/145135 |
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