GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila

Rahman, Ashrafur and Lőrincz, Péter and Gohel, Raksha and Nagy, Anikó and Csordás, Gábor and Juhász, Gábor (2022) GMAP is an Atg8a-interacting protein that regulates Golgi turnover in Drosophila. CELL REPORTS, 39 (9). No. 110903. ISSN 2211-1247

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Abstract

Selective autophagy receptors and adapters contain short linear motifs called LIR motifs (LC3-interacting re- gion), which are required for the interaction with the Atg8-family proteins. LIR motifs bind to the hydrophobic pockets of the LIR motif docking site (LDS) of the respective Atg8-family proteins. The physiological signif- icance of LDS docking sites has not been clarified in vivo. Here, we show that Atg8a-LDS mutant Drosophila flies accumulate autophagy substrates and have reduced lifespan. Using quantitative proteomics to identify the proteins that accumulate in Atg8a-LDS mutants, we identify the cis-Golgi protein GMAP (Golgi microtu- bule-associated protein) as a LIR motif-containing protein that interacts with Atg8a. GMAP LIR mutant flies exhibit accumulation of Golgi markers and elongated Golgi morphology. Our data suggest that GMAP medi- ates the turnover of Golgi by selective autophagy to regulate its morphology and size via its LIR motif-medi- ated interaction with Atg8a.

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