Introducing 77Se Spectroscopy to Analyzing Galectin–Ligand Interaction

Raics, Mária and Timári, István and Szilágyi, László and Gabius, Hans-Joachim and E. Kövér, Katalin (2022) Introducing 77Se Spectroscopy to Analyzing Galectin–Ligand Interaction. In: Galectins: Methods and Protocols, Methods in Molecular Biology. Humana, New York, pp. 1-19. (In Press)

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Abstract

Their emerging nature as multifunctional effectors explains the large interest to monitor glycan binding to galectins and to define bound-state conformer(s) of their ligands in solution. Basically, NMR spectroscopy facilitates respective experiments. Towards developing new and even better approaches for these purposes, extending the range of exploitable isotopes beyond1H,13C, and15N offers promising perspectives. Having therefore prepared selenodigalactoside and revealed its bioactivity as galectin ligand, monitoring of its binding by77Se NMR spectroscopy at a practical level becomes possible by setting up a 2D1H,77Se CPMG-HSQBMC experiment including CPMG-INEPT long-range transfer. This first step into applying77Se as sensor for galectin binding substantiates its potential for screening relative to inhibitory potencies in compound mixtures and for achieving sophisticated epitope mapping. The documented strategic combination of synthetic carbohydrate chemistry and NMR spectroscopy prompts to envision to work with isotopically pure77Se-containing β-galactosides and to build on the gained experience with77Se by adding19F as second sensor in doubly labeled glycosides. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

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