Bánfi, Renáta and Pohner, Zsuzsanna and Szabó, Attila and Herczeg, Gábor and Kovács, M. Gábor and Márialigeti, Károly and Vajna, Balázs (2021) Succession and potential role of bacterial communities during Pleurotus ostreatus production. FEMS MICROBIOLOGY ECOLOGY, 97 (10). ISSN 0168-6496
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Abstract
There is an increasing interest in studying bacterial-fungal interactions (BFIs), also the interactions of Pleurotus ostreatus, a model white-rot fungus and important cultivated mushroom. In Europe, P. ostreatus is produced on a wheat straw-based substrate with a characteristic bacterial community, where P. ostreatus is exposed to the microbiome during substrate colonisation. This study investigated how the bacterial community structure was affected by the introduction of P. ostreatus into the mature substrate. Based on the results obtained, the effect of the presence and absence of this microbiome on P. ostreatus production in an experimental cultivation setup was determined. 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) and amplicon sequencing revealed a definite succession of the microbiome during substrate colonisation and fruiting body production: a sharp decrease in relative abundance of Thermus spp. and Actinobacteria, and the increasing dominance of Bacillales and Halomonas spp. The introduced experimental cultivation setup proved the protective role of the microbial community against competing fungi without affecting P. ostreatus growth. We could also demonstrate that this effect could be attributed to both living microbes and their secreted metabolites. These findings highlight the importance of bacterial-fungal interactions during mushroom production.
Item Type: | Article |
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Uncontrolled Keywords: | Pleurotus ostreatus; T-RFLP; amplicon sequencing; bacterial community structure; bacterial-fungal interactions; succession and spatial heterogeneity |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 29 Sep 2022 05:41 |
Last Modified: | 29 Sep 2022 05:41 |
URI: | http://real.mtak.hu/id/eprint/150434 |
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