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Isolation and identification of carotenoid-producing yeast and evaluation of antimalarial activity of the extracted carotenoid(s) against P. falciparum

Sinha, Sweta and Chakrabarti, Amrita and Singh, Gunjan and Kumar, Kukkala Kiran and Gaur, Naseem A. and Arora, Anju and Singh, Kamalesh Narain and Singh, Shailja and Paul, Debarati (2021) Isolation and identification of carotenoid-producing yeast and evaluation of antimalarial activity of the extracted carotenoid(s) against P. falciparum. Biologia Futura, 72 (3). pp. 325-337. ISSN 2676-8615 (print), 2676-8607 (online)

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Abstract

Plasmodial resistance to a variety of plant-based antimalarial drugs has led toward the discovery of more effective antimalarial compounds having chemical or biological origin. Since natural compounds are considered as safer drugs, in this study, yeast strains were identified and compared for the production of carotenoids that are well-known antioxidants and this metabolite was tested for its antiparasitic activity. Plasmodium falciparum 3D7 strain was selected as the target parasite for evaluation of antimalarial activity of yeast carotenoids using in vitro studies. Data were analyzed by FACS (fluorescence-activated cell sorter) and counted via gold standard Giemsa-stained smears. The extracted yeast carotenoids showed a profound inhibitory effect at a concentration of 10–3 µg/µl and 10−4 µg/µl when compared to β- carotene as control. SYBR Green1 fluorescent dye was used to confirm the decrease in parasitaemia at given range of concentration. Egress assay results suggested that treated parasite remained stalled at schizont stage with constricted morphology and were darkly stained. Non-toxicity of carotenoids on erythrocytes and on human liver hepatocellular carcinoma cells (HepG2 cells) was shown at a given concentration. This report provides strong evidence for antimalarial effects of extracted yeast carotenoids, which can be produced via a sustainable and cost-effective strategy and may be scaled up for industrial application.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Depositing User: Zsolt Baráth
Date Deposited: 14 Oct 2022 11:06
Last Modified: 17 Oct 2022 06:29
URI: http://real.mtak.hu/id/eprint/151712

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