Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

Sárvári, Éva and Gellén, Gabriella and Sági-Kazár, Máté and Schlosser, Gitta and Solymosi, Katalin and Solti, Ádám (2022) Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE. PLANT METHODS, 18 (1). ISSN 1746-4811

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Abstract

Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE is a widely used, convenient and time efficient method to separate thylakoid complexes and study their composition, abundance, and interactions. Previous analyses unravelled multiple monomeric and dimeric/oligomeric thylakoid complexes but, in certain cases, the separation of complexes was not proper. Particularly, the resolution of super- and megacomplexes, which provides important information on functional interactions, still remained challenging.

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