Rai, Abhilasha and Jha, Manindra Nath and Singh, Devendra and Thapa, Shobit and Chaurasia, Sanjeet Kumar and Jha, Gopaljee (2022) Detection of endophytic association between Aeschynomene nodulating Bradyrhizobium sp. and traditional Desariya rice roots under rice-Aeschynomene ecosystem of chaur land, Bihar, India. BIOLOGIA FUTURA : A QUARTERLY OF THE HUNGARIAN ACADEMY OF SCIENCES, 73 (1). pp. 95-105. ISSN 2676-8615 (print); 2676-8607 (online)
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Abstract
Engineering diazotrophic rice having either an integral component of diazotrophic microbes or placing microbial origin nif gene to the rice plant is the dream of biotechnologist. Rice-Aeschynomene ecosystem of pristine chaur land provides a suitable niche to search Rhizobium endophytes in rice. Accordingly, the work was initiated to search suitable endophytic Rhizobium strain for artificial symbiosis within the roots of Desariya rice and its source through morphological, biochemical and molecular approaches. Detection of Acetylene reduction assay (ARA) activity in sterilized Desariya rice root confirmed the presence of putative diazotrophic endophytes in rice root. Isolates from Aeschynomene aspera L. nodulating and Desariya rice endophytic Rhizobium were evaluated for growth, IAA, morphological and biochemical features. Carbon profiling pattern of both these isolates indicated that Desariya rice endophytic Rhizobium has its similarity with Aeschynomene aspera L. nodulating Rhizobium. 16S rRNA gene sequencing confirmed the presence of endophytic Bradyrhizobium sp. in Desariya rice roots and its similarity with Aeschynomene aspera L. nodulating Bradyrhizobium. Desariya rice Bradyrhizobium may be an ideal candidate in the future for creating artificial symbiosis in rice due to its similarity with Aeschynomene aspera L. Bradyrhizobium.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > Q1 Science (General) / természettudomány általában Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia |
Depositing User: | Zsolt Baráth |
Date Deposited: | 17 Oct 2022 08:58 |
Last Modified: | 17 Oct 2022 08:58 |
URI: | http://real.mtak.hu/id/eprint/151801 |
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