Benzaldehyde-induced developmental genotoxicity triggers both neural and non-neuronal cells including the cells of immunity in Drosophila melanogaster

Ghosh, Swapan Kumar and Chatterjee, Tridip and Mitra, Sagarika and Chakravarty, Amit and Chakravarty, Sudipa and Basak, Ashim Kumar (2022) Benzaldehyde-induced developmental genotoxicity triggers both neural and non-neuronal cells including the cells of immunity in Drosophila melanogaster. BIOLOGIA FUTURA : A QUARTERLY OF THE HUNGARIAN ACADEMY OF SCIENCES, 73 (2). pp. 245-257. ISSN 2676-8615 (print); 2676-8607 (online)

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Abstract

Although benzaldehyde, an aromatic aldehyde, has been declared safe for uses in food, conflicting reports exist regarding its genotoxic and cytotoxic potentials in organisms. Our present study is the first attempt to evaluate the effects of exposure of benzaldehyde on the entire course of development of a eukaryote model organism, Drosophila melanogaster. Total time required for the initial appearance of the third instar larvae, pupae and adults increased dose dependently with the increasing dietary concentration of benzaldehyde. Exposure of flies to each concentration of benzaldehyde caused dose-dependent and significant reductions in the population of pupae and young adults of the fly. Developmental inhibition was associated with dose dependent and significant structural aberrations of larval polytene chromosomes like ectopic pairing, inversion, fusion, etc., and deformities of hemocytes and neuroblasts and death of hemocytes. As much as 34% (SD ± 1.76)-52% (SD ± 1.7) and 18% (SD ± 2.5)-40% (SD ± 3.38) hemocytes and neuroblasts, respectively, underwent nuclear deformations in response to dietary exposures of flies to BA 100–1000 mg/l. Moreover, 16% (SD ± 0.52)-31% (SD ± 1.97) and 19% (SD ± 0.3)-33% (SD ± 1.78) hemocyte mortalities in response to BA 100–1000 mg/l were determined by two cell viability assays. Thus our study revealed that benzaldehyde was genotoxic to Drosophila melanogaster larvae that might be responsible for larval cell death and their subsequent developmental retardation. As this fly possesses substantial genetic homology with human, possibility of developmental inhibition of the later due to exposure of this chemical during pregnancy may not be ruled out.

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