Expression and estrogen-dependent up-regulation of Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) ion channels in the rat endometrium

Pohóczky, Krisztina and Kun, József and Szalontai, Bálint and Kovács, Krisztina and Garai, János and Garami, András and Perkecz, Anikó and Helyes, Zsuzsanna (2014) Expression and estrogen-dependent up-regulation of Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) ion channels in the rat endometrium. In: Joint Meeting of the Federation of European Physiological Societies (FEPS) and the Hungarian Physiological Society., 2014. augusztus 27-30., Budapest.

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Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) cation channels localized predominantly on capsaicin-sensitive peptidergic sensory nerves play essential roles in pain, hyperalgesia and neurogenic inflammation. They are activated by a variety of noxious stimuli, chemical irritants and cold or heat, respectively. Besides sensory nerves, both receptors have been described on epithelial and immune cells. Estrogen-induced TRPV1 up-regulation in the human uterus suggests its potential involvement in pain during the reproductive cycle. Since there are no data regarding TRPA1 expression in the endometrium and little is known about TRPV1 regulation, we investigated estrogen- and progesterone-dependent alterations of these channels in the rat endometrium. Different groups of sexually premature 4-week-old and adult 4-month-old female rats were treated with subcutaneously implanted wax pellets containing synthetic estrogen analog diethylstilbestrol (DES, 100 µg), progesterone (4 mg) and their combination for 8 or 12 days, respectively. Ovariectomy was performed in separate groups of 4-month-old animals (n=5/group). TRPA1 and TRPV1 mRNA levels were measured in the endometrium layer with quantitative PCR, while the localization of the receptor proteins was determined with immunohistochemistry on paraffin-embedded uterus sections. Both TRPA1 and TRPV1 were detected in the rat endometrium at mRNA and protein levels as well, showing their remarkable local, non-neuronal expression. DES treatment resulted in a 5-fold and 7-fold significant up-regulation of TRPV1 mRNA in young and adult rats, respectively, which were absent if progesterone was added simultaneously. DES also induced significant elevation of TRPA1 mRNA in both groups. Progesterone by itself did not alter the levels of either channel in either group. In young rats, weak TRPV1 and A1 staining were observed in the epithelium, while in adult animals it was detected in the stroma and the glands with weak expression in the epithelium. Further investigations are in process to elucidate the functions of TRPA1 and TRPV1 in conditions related to pain and inflammation. SROP-4.2.2.A-11/1/KONV-2012-0024

Item Type: Conference or Workshop Item (Poster)
Subjects: Q Science / természettudomány > QP Physiology / élettan
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: Dr. Andras Garami
Date Deposited: 22 Sep 2014 09:43
Last Modified: 22 Sep 2014 09:43

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