Tying Up a Loose End: On the Role of the C‐Terminal CCHHRAG Fragment of the Metalloregulator CueR

Balogh, Ria K. and Gyurcsik, Béla and Jensen, Mikael and Thulstrup, Peter W. and Köster, Ulli and Christensen, Niels Johan and Jensen, Marianne L. and Hunyadi-Gulyás, Éva and Hemmingsen, Lars and Jancsó, Attila (2022) Tying Up a Loose End: On the Role of the C‐Terminal CCHHRAG Fragment of the Metalloregulator CueR. CHEMBIOCHEM, 23 (16). ArtNo: e202200290. ISSN 1439-7633

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Abstract

The transcriptional regulator CueR is activated by the binding of CuI, AgI, or AuI to two cysteinates in a near-linear fashion. The C-terminal CCHHRAG sequence in Escherichia coli CueR present potential additional metal binding ligands and here we explore the effect of deleting this fragment on the binding of AgI to CueR. CD spectroscopic and ESI-MS data indicate that the high AgI-binding affinity of WT-CueR is significantly reduced in Δ7C-CueR.[111 Ag PAC spectroscopy demonstrates that the WT-CueR metal site structure (AgS2) is conserved, but less populated in the truncated variant. Thus, the function of the C-terminal fragment may be to stabilize the two-coordinate metal site for cognate monovalent metal ions. In a broader perspective this is an example of residues beyond the second coordination sphere affecting metal site physicochemical properties while leaving the structure unperturbed.

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