EMS induced SNP changes led to mutation of Wx protein in common wheat

Lan, J. and Li, Y. and Xu, K. and Zhang, X. and Tang, H. and Qi, P. and Ma, J. and Wang, J. and Chen, G. and Pu, Z. and Li, W. and Li, Z. and Harwood, W. and Lan, X. and Deng, M. and Wei, Y. and Zheng, Y. and Jiang, Q. (2020) EMS induced SNP changes led to mutation of Wx protein in common wheat. CEREAL RESEARCH COMMUNICATIONS, 48 (2). pp. 233-238. ISSN 0133-3720 (print), 1788-9170 (online)

Text CRC_48_233-238.pdf Restricted to Repository staff only Download (587kB)

Abstract

Three EMS mutant wheat lines with varied mobility of Wx-A1 and Wx-B1 were identifed. In the mutant lines M2-97-8 and M2-104-2, Wx-B1 showed slightly faster mobility compared to those of wild line, and Wx-A1 in the mutant line M2-101-1 has a greatly faster mobility, which co-migrates with Wx-D1. The results of gene sequencing indicated that SNP mutations appeared within exon of Wx-1 and further resulted in the mutation of amino acid residues in the mutant lines. The prediction analysis showed that the isoelectric point of the mutants M2-97-8 and M2-104-2 was more basic than that of the wild type while the isoelectric point of M2-101-1 was not changed; and the Waxy protein of the three mutations were all around 66 kDa. The SDS-PAGE results revealed that the mobility variation of Wx-B1 of M2-97-8 and M2-104-2 is due to the change of isoelectric point, while M2-101-1 may be caused by variation or modifcation of protein structure but not by the change of isoelectric point. The prokaryotic expression results further indicated that these mutated Wx-A1 and Wx-B1 genes migrated faster than those of wild Wx-A1 and Wx-B1 and were consistent with the results of molecular weight prediction. Although, the increased or decreased contents of total starch and amylose were observed in Wx-A1 or Wx-B1mutant, the correlation between the contents of total starch and amylose and the mutant Wx-A1 and Wx-B1 subunits cannot be determined. These mutants can not only increase the polymorphism of Waxy gene to investigate the function of these alleles, but also be valuable for wheat quality breeding.

Actions (login required)

Edit Item