Reconsidering the direct cross approach for gene transfer from Aegilops tauschii to hexaploid wheat using diversified D genome donors

Cambay, S. R. and Bains, N. S. and Sandhu, S. K. and Puja, Srivastava (2021) Reconsidering the direct cross approach for gene transfer from Aegilops tauschii to hexaploid wheat using diversified D genome donors. CEREAL RESEARCH COMMUNICATIONS, 49 (1). pp. 125-131. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

The direct cross-hybridization approach between 16 Aegilops tauschii (AT) and 10 bread wheat (W) lines was carried out in reciprocal directions. W (♀) x AT (♂) yielded no embryo formation whereas embryo formation ranging from 34.09 to 43.33% was obtained in AT (♀) x W (♂) crosses in all 160 combinations. The major bottleneck of low plant regeneration in AT (♀) x W (♂) crosses was cleared by adopting detached tiller culture and embryo rescue medium adjuvants such as casein hydrolysate (200 mg/L) and activated charcoal (2 g/L). The regeneration frequency as a result of these two interventions increased from 3.61 to 19.29 per cent. Two innovative methods of chromosome doubling included in situ colchicine (0.05%) treatment with cotton swab method and pre-regeneration colchicine treatment (0.1 and 0.2%) to pollinated ovaries were found to be effective. The findings of the study make successful exploitation of direct cross approach (AT (♀) x W (♂)) possible in wheat improvement.

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