Genotype-specific patterns of physiological and antioxidative responses in barley under salinity stress

Ouertani, Rim Nefissi and Jardak, Rahma and Ben Chikha, Mariem and Ben Yaala, Wiem and Abid, Ghassen and Karmous, Chahine and Hamdi, Zohra and Mejri, Samiha and Jansen, Robert K. and Ghorbel, Abdelwahed (2022) Genotype-specific patterns of physiological and antioxidative responses in barley under salinity stress. CEREAL RESEARCH COMMUNICATIONS, 50 (4). pp. 851-863. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Using reliable salt tolerance markers is a key component in barley breeding programs. In this study, physiological and antioxidative markers of two Tunisian barley salinity tolerance contrasting genotypes Boulifa (B) and Manzel Habib (MH) were assessed at 0, 3, 6 and 9 days of 200 mM salt treatment. Salinity caused decrease in growth, degraded photosynthetic activity and reduced water-holding capacity in both genotypes with more pronounced negative effects in the salt-sensitive (MH) compared to the salt-tolerant (B) genotype. On the other hand, the lower oxidative damage in B compared to MH under salt stress could be explained by higher activities of antioxidant enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and glutathione reductase (GR). Additionally, a genotype-specific pattern of enzyme activity and corresponding gene expression was revealed in the two barleys under salt stress. In this context, a positive correlation was noted for the SOD. On the other hand, multivariate analysis marked SOD and APX as the most discriminating factors between both stressed genotypes. Our findings could be considered for selection in breeding programs for salt stress tolerance in barley.

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