Phylogenetic diversity of barley- and wheat-specific forms of Wheat dwarf virus in Turkey

Morca, Ali Ferhan and Coskan, Sevgi and Akbas, Birol (2022) Phylogenetic diversity of barley- and wheat-specific forms of Wheat dwarf virus in Turkey. CEREAL RESEARCH COMMUNICATIONS, 50 (4). pp. 1029-1036. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Wheat dwarf virus (WDV) has caused an epidemic in many cereal production areas of Turkey in recent years. There is no current literature data on this important virus in the country. This study presents the extensive molecular characterization of WDV isolates causing serious problems in the Central Anatolia Region between 2019 and 2020. Of the 51 locations, 44 locations (25 barley + 19 wheat) were found to be infected with WDV and 7 locations (1 barley + 6 wheat) were WDV-negative by PCR (Polymerase chain reaction). To differentiate the wheat- and barley-specific (WDV-W and WDV-B) forms of WDV, PCR tests were also performed. Eleven locations were identified as WDV-W, and 33 locations were identified as WDV-B. Although two Turkish isolates in WDV-B specific form have been reported in previous studies, isolates belonging to WDV-W specific form were identified for the first time by this study. Partial sequences of randomly selected 16 isolates (8 WDV-W and 8 WDV-B) were realized and deposited in GenBank. The phylogenetic tree was generated by the neighbor-joining method, and 16 isolates were clustered as follows: two isolates in WDV-A, six isolates in WDV-B, and eight isolates in WDV-E. To our knowledge, this study is the first report of B and E strains of WDV in Turkey. This is the first comprehensive study presenting both specific form prevalence and the strains of WDV in Turkey.

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