Evaluation of differentially expressed genic marker linked resistance gene analog (RGAs) effective at seedling stage against spot blotch in wheat

Yadav, Aakash and Chandra, Tilak and Kumar, Sundeep (2023) Evaluation of differentially expressed genic marker linked resistance gene analog (RGAs) effective at seedling stage against spot blotch in wheat. CEREAL RESEARCH COMMUNICATIONS, 51 (1). pp. 155-162. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Spot blotch caused by Bipolaris sorokiniana is an economically significant disease of wheat that can cause yield losses up to 70%. It is a disease of great concern, especially in South Asia and Latin American countries, where warm-humid conditions persist during the wheat cropping season. In eastern parts of India, it is a major issue which sometimes occurs up to epidemic proportions. Lacking suitable resistant genes in high-yielding cultivars further add to the problem and reason of huge yield losses. Resistant gene analog (RGAs) are a class of resistant genes with conserved modules provides resistance against such devastating pathogens. They resist a plethora of disease-causing pathogen and orchestrated a response via activating numerous downstream cascades of pathogenesis ultimately leads to survival under biotic encounter. Here, we postulated that expression of these genes are modulated upon infection in resistant cultivar (BHU-35) compared to known susceptible cultivar i.e., Sonalika at seedling stage upon artificial infestation of two diverse isolates PUSA2 and HD3069, individually. Moreover, these sequences act as a genic marker in the cultivar and could be used to differentiate resistant from the susceptible ones. This study about RGAs genes provides a novel genic marker-assisted differential gene expression in contrasting cultivars and thus, use to generate resistance in susceptible cultivars by stacking these classes of genes using molecular breeding/biotechnological approaches. An increasing proportion of such devastating diseases in wheat-growing regions necessitated the development of resistant varieties to tackle the disease.

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