Development of in vitro pollen germination protocol for recalcitrant triticale pollen (X Triticosecale Wittmack)

Jayaprakash, P. and Peter, John and Shajitha, P. and Balaji, V. and Nisha, R. and Geetha, M. and Vikas, V. K. and Sivasamy, M. (2023) Development of in vitro pollen germination protocol for recalcitrant triticale pollen (X Triticosecale Wittmack). CEREAL RESEARCH COMMUNICATIONS, 51 (1). pp. 189-196. ISSN 0133-3720 (print), 1788-9170 (online)

Text CRC_51_189-196.pdf - Published Version Restricted to Repository staff only Download (1MB)

Abstract

An in vitro pollen germination protocol for Triticale, the man-made cereal, has been reported. Triticale pollen may be classified as recalcitrant pollen as it is difficult to germinate on artificial medium. Using this pollen germination medium (PGM), viability and pollen vigour of triticale lines may be tested within 10–15 min. PGM used for triticale consisted of 19% maltose, 15% polyethylene glycol 4000, 60 mgl−1 boric acid, 20 mgl−1 calcium nitrate, 80 mgl−1 potassium nitrate, 160 mgl−1 magnesium sulphate and 1% agar. This PGM has been altered slightly which supported pollen germination of other genotypes. This complex medium contains many inorganic salts but in low concentration. The PGM showed > 93% pollen germination with intact pollen tubes at 17 ± 1 °C and pH 6.8. During the flowering period, a pollen sterility of less than 10% was recorded. Triticale pollen was viable more than 1.45 h which is much longer time as compared to wheat. This tool would be useful in triticale breeding especially in wide crosses with wheat or rye.

Actions (login required)

Edit Item