Effects of different modes and intensities of exercise on longevity proteins in middle-aged mouse skeletal muscle

Inoue, Shota and Matsuura, Kyohei and Eguchi, Daisuke and Wakayama, Masahiro and Ono, Kosuke (2023) Effects of different modes and intensities of exercise on longevity proteins in middle-aged mouse skeletal muscle. PHYSIOLOGY INTERNATIONAL, 110 (2). pp. 150-159. ISSN 2498-602X

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Abstract

Physical exercise represents one of the most effective approaches to anti-aging. The goal of this study was to verify the effects of different modes and intensities of exercise on longevity proteins in the skeletal muscle in midlife. Middle-aged mice were trained in aerobic or resistance exercise for 8 weeks, and the changes in sirtuin 1 (SIRT1), adenosine monophosphate-activated kinase (AMPK), and mammalian target of rapamycin (mTOR) pathways in the skeletal muscle were evaluated by western blotting. Long-term exercise had no effects on skeletal muscle SIRT1 abundance, whereas high-intensity aerobic exercise increased AMPK phosphorylation and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). Low-intensity resistance exercise facilitated Akt/mTOR/p70 ribosomal protein kinase S6 (p70S6K) signaling but did not induce muscle hypertrophy. Conversely, high-intensity resistance exercise stimulated muscle hypertrophy without phosphorylation of mTOR signaling-related proteins. These results suggest the importance of setting exercise modes and intensities for anti-aging in midlife.

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