Alexandrova, Alexandra Sashova and Pencheva, Daniela Rosenova and Setchanova, Lena Petrova and Gergova, Raina Tsvetanova (2022) Association of pili with widespread multidrug- resistant genetic lineages of non-invasive pediatric Streptococcus pneumoniae isolates. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 69 (3). pp. 177-184. ISSN 1217-8950
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Abstract
The study aimed to evaluate the presence of pili in non-invasive pediatric pneumococcal isolates and to elucidate possible links with genetic lineages, serotypes, and antimicrobial resistance. We examined 147 Streptococcus pneumoniae isolates from children with respiratory tract infections and acute otitis media. Serotyping was performed by latex agglutination and capsule swelling reaction. Serogroup 6 was subjected to PCR-serotyping. Minimum inhibitory concentrations were determined according to EUCAST breakpoints. PCRs for rlrA and pitB genes were performed to detect a presence of type 1 and type 2 pili. MLST was conducted to define the clonal structure of the piliated strains. Almost all children (96.5%) were vaccinated with the pneumococcal conjugate vaccine PCV10. We detected 76.8% non-PCV10 - serotypes (NVTs) and 14.3% PCV10 serotypes. The predominant serotypes were NVTs: 19A (14.3%), 6C (12.2%), 3 (9.5%), 15A (7.5%) and 6A (6.8%). PI-1 was detected among 10.9% non-PCV10 serotypes 6A, 6C, and 19A and 6.1% PCV10 serotypes 19F and 23F. Type 2 pili were not found in the studied population. High levels of antimicrobial nonsusceptibility to erythromycin (58.5%), oral penicillin (55.8%), clindamycin (46.9%), trimethoprim-sulfamethoxazole (45.6%), tetracycline (39.5%) and ceftriaxone (16.3%) were revealed. The multidrug-resistant strains (MDR) were 55.1%. MLST represented 18 STs and three CCs among the piliated pneumococci: CC386, CC320, and CC81. More than half of the piliated strains (56.0%) belonged to successfully circulating international clones. PI-1 was associated mainly with MDR 6A, 6C, 19A, 19F, and 23F isolates from the widespread CC386, CC320, and CC81.
| Item Type: | Article |
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| Additional Information: | Department of Medical Microbiology, Medical Faculty, Medical University of Sofia, 1, G.Sofiiski Boul., Sofia, 1431, Bulgaria Department of Medical Chemistry and Biochemistry, Molecular Medicine Center, Medical Faculty, Medical University of Sofia, 2, Zdrave str., Sofia, 1431, Bulgaria Export Date: 31 January 2023 Correspondence Address: Alexandrova, A.S.; Department of Medical Microbiology, 1, G.Sofiiski Boul., Bulgaria; email: alex.alexandrova1@gmail.com |
| Uncontrolled Keywords: | serotype; Streptococcus pneumoniae; ANTIMICROBIAL RESISTANCE; Clonality; pilus; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 08 Aug 2023 07:10 |
| Last Modified: | 30 Sep 2023 23:15 |
| URI: | http://real.mtak.hu/id/eprint/171033 |
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