Antimicrobial resistance patterns and virulence gene profiles of Salmonella enteritidis and Salmonella typhimurium recovered from patients with gastroenteritis in three cities of Iran

Sharahi, Javad Yasbolaghi and Hashemi, Ali and Mousavi, Seyyed Mohammad Javad and Pournajaf, Abazar and Bidhendi, Soheila Moradi (2022) Antimicrobial resistance patterns and virulence gene profiles of Salmonella enteritidis and Salmonella typhimurium recovered from patients with gastroenteritis in three cities of Iran. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 69 (4). pp. 323-331. ISSN 1217-8950

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Abstract

This study evaluated distribution of virulence factors and antibiotic resistance in clinical isolates of Salmonella enteritidis and Salmonella typhimurium in three cities of Iran. Altogether 48 S. enteritidis and S. typhimurium isolates were collected from patients at certain Iranian hospitals between May 2018 and September 2021. Antimicrobial susceptibility testing was performed by disk diffusion and broth microdilution methods. The presence of antibiotic-resistance genes (blaTEM, blaSHV, blaCTX- M, blaNDM, strA, strB, aadA1, tetA, tetB, floR, sul1, sul2, dfrA), integrons (classe 1 and 2), and virulence-associated genes (invA, stn, sopB, spvC, rck, phoPQ) was investigated by PCR and sequencing. Antimicrobial agents like trimethoprim-sulfamethoxazole and imipenem represent highly efficient agents with 97% susceptibility. S. enteritidis and S. typhimurium exhibited high resistance to ciprofloxacin ( n 5 20, 71.43%) and ceftazidime (n 5 9, 45%), respectively. Overall, 3 (6.25%), 13 (27.08%), and 6 (12.5%) isolates were divided into strong, moderate, and weak biofilm producers, respectively. Moreover, blaCTX- M, blaTEM, blaSHV, sul1, sul2, tetA, tetB, floR, strA, and strB resistant genes were detected in 10 (20.8%), 5 (10.4%), 1 (2.08%), 7 (14.58%), 1 (2.08%), 3 (6.25%), 2 (4.1%), 1 (2.08%), 2 (4.1%), 2 (4.1%), respectively. Furthermore, 7 (14.58%) strains had classe 1 integron. All tested S. enteritidis strains had invA and sopB, and all S. typhimurium strains had invA and phoPQ. However, spvC remained undetected in all isolates. Extensive surveillance and efficient control measures against infection help to stop the upsurge of various antibiotic-resistant isolates.

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