Asgharzadeh, Sajjad and Zadeh, Rezvan Golmoradi and Moghadam, Majid Taati and Eraghiye, Hamed Farahani and Kalani, Behrooz Sadeghi and Jazi, Faramarz Masjedian and Mirkalantari, Shiva (2022) Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 69 (4). pp. 314-322. ISSN 1217-8950
|
Text
1588-2640-article-p314.pdf Download (1MB) | Preview |
Abstract
Escherichia coli ST131 is a pandemic clone with high antibiotic resistance, and it is a major causative agent of urinary tract infection (UTI) and bloodstream infections. This study evaluated the distribution and expression of virulence genes and genotyping of E. coli O25b/ST131 by Multi-locus variable number tandem repeat analysis (MLVA) method among UTI in patients at Tehran hospitals, Iran. A total of 107 E. coli isolates were collected from UTI patients. Polymerase chain reaction (PCR) amplification of the pabB gene was used to identify E. coli O25b/ST131 and the prevalence of sat and hlyA virulence genes was also analyzed. The microtiter method quantified biofilm formation ability in E. coli O25b/ST131. The Real-Time PCR (qRT-PCR) was performed to evaluate the expression of sat and hlyA genes. Finally, MLVA was performed for E. coli O25b/ST131 genotyping by targeting seven tandem repeats. SPSS-16 software was used for statistical analysis. Molecular study showed that 71% of isolates carried the pabB gene and were considered E. coli O25b/ST131 strains. Also, 45.8% and 17.8% of isolates carried sat and hlyA genes, respectively. The 57.9% isolates had biofilm formation ability. Expression of the studied virulence genes showed an increase in strong biofilm producing E. coli O25b/ST131 strains. A total of 76 (100%) E. coli O25b/ST131 strains were typed by the MLVA method. High prevalence of E. coli O25b/ST131 isolates in UTI patients can be a serious warning to the treatment due to the high antibiotic resistance rate, expression of virulence genes, and biofilm formation.
Item Type: | Article |
---|---|
Additional Information: | Funding Agency and Grant Number: [18525] Funding text: This study was financially supported by a research grant (No.18525) for an M.Sc thesis at Iran University of Medical Sciences (Tehran, Iran), for which we are very grateful |
Uncontrolled Keywords: | immunology; URINARY-TRACT-INFECTIONS; ANTIBIOTIC-RESISTANCE; E. coli ST131; ST131; MLVA; Biofilm production; |
Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 14 Aug 2023 05:07 |
Last Modified: | 31 Dec 2023 00:17 |
URI: | http://real.mtak.hu/id/eprint/171403 |
Actions (login required)
![]() |
Edit Item |