Kizilyildirim, Suna and Koksal, Fatih and Guneri, Cansu Onlen (2022) Distribution of emm, superantigen and other virulence genotypes and detection of phylogenetic relationships in group A streptococcal isolates. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 69 (4). pp. 290-296. ISSN 1217-8950
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Abstract
Group A streptococci are important pathogens with various virulence factors, such as M protein, superantigens, hemolysins, deoxyribonuclease, and proteases. The aims of this study are to investigate the detection of emm genotypes and other virulence genes, such as SAgs, DNase, protease, antibiotic resistance, and phylogenetic relationships in GAS strains isolated from clinical samples. Test strains were obtained from Cukurova University Balcali Hospital and regional hospitals in Adana province. The M proteins were detected by sequence analysis of emm genes. SAgs and other virulence gene profiles were determined using the Multiplex-PCR method. The antibiotic susceptibility of the isolates was performed by the disc diffusion method and evaluated according to CLSI criteria. The PFGE method was used to determine the clonal relationship between the strains. The emm gene was positive in 86 isolates. The most common emm genotypes were emm28 (22%), emm1 (18.6%), emm12 (13.9%), and emm3 (11.6%). Also, the most common virulence genes were speG (58.1%), speC (56.9%), sdaB (53.4%), and mac (53.4%). The rates of resistance to erythromycin, clindamycin, levofloxacin, ciprofloxacin and telithromycin were 19.8%, 16.3%, 4.7%, 3.5%, and 3.5%, respectively. As a result, additional regional studies on the detection and prevalence of GAS virulence factors in Turkey are required. We believe that this study will provide valuable information for epidemiological studies on emm sequences, Sags, and other virulence factors of Streptococcus pyogenes in Turkey.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Cukurova University [TDK-2014-3348] Funding text: We would like to acknowledge that Cukurova University financially supported this study (TDK-2014-3348). We thank Prof. Dr. Androulla Efstratiou and Dr. Victoria Jane Chalker for their help in implementing the emm protocol. |
| Uncontrolled Keywords: | immunology; Pathogenesis; EPIDEMIOLOGY; PYOGENES; NONINVASIVE INFECTIONS; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 15 Aug 2023 07:07 |
| Last Modified: | 31 Dec 2023 00:17 |
| URI: | http://real.mtak.hu/id/eprint/171467 |
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