Temperate hyper-saline ecosystems induce spatial distribution and halo-thermotolerance potential in indigenous cultivable bacterial community

Phulpoto, Irfan Ali and Jakhrani, Bakhtiar Ali and Panhyar, Asif Ali and Phulpoto, Anwar Hussain and Shaikh, Sadam Hussain and Channa, Najeebullah and Kanhar, Nisar Ahmed and Qazi, Muneer Ahmed (2022) Temperate hyper-saline ecosystems induce spatial distribution and halo-thermotolerance potential in indigenous cultivable bacterial community. COMMUNITY ECOLOGY, 23 (3). pp. 337-347. ISSN 1585-8553 (print); 1588-2756 (online)

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Abstract

Extreme environmental conditions like high salinity and temperature are continuously affecting fertile lands and trigger an ecological imbalance, thereby inducing indigenous microbial communities for adaptation to such extreme environments. Present study relates with the isolation and characterization of halo-thermotolerant bacteria (HTTB) from hyper-saline areas of districts Kashmore and Khairpur, Sindh, Pakistan. The isolated bacteria were initially screened for their tolerance to elevated salt concentrations and extreme temperature ranges. Out of twenty-six bacterial isolates, six bacteria from district Kashmore (60%) and thirteen bacterial isolates from district Khairpur (81.25%) tolerated NaCl concentration up to 15–17% (w/v), and grown efficiently when incubated in the temperature ranges 55–65 °C. The principle component and multivari- ate cluster analyses revealed three different clusters of HTTB bacteria based on their tolerance and spatial distribution. In general, the hyper-saline environments of district Kashmore harbored more HTTB than Khairpur, but individually there was extreme halotolerance and thermotolerance among Khairpur isolates. Finally, the PCR-based molecular typing of 16S rRNA genes revealed, majority of the isolates were identified to be various strains of genus Bacillus, i.e., Bacillus subtilis, B. licheniformis, B. aerius, B. vallismortis, B. aquimaris and B. flexus. Only one bacterial strain, i.e., KJ1WB, was identified as Pseudomonas pseudoalcaligenes. These HTTB bacterial strains may extend significant potential for future applications like environmental, biotechnological and enormous industrial due to their valuable enzymatic profiles and metabolite repositories.

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