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Statistical optimization of media components for production of extracellular lipase from edible mushroom Cantharellus cibarius

Dutta, Bhramar and Shamekh, Salem and Deska, Jan and Bandopadhyay, Rajib (2022) Statistical optimization of media components for production of extracellular lipase from edible mushroom Cantharellus cibarius. BIOLOGIA FUTURA, 73 (3). pp. 315-325. ISSN 2676-8615 (print); 2676-8607 (online)

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Abstract

Cantharellus cibarius is a wild edible mushrooms and considered as a plethora of compounds with potential biotechnological applications. This study highlighted the utilization of C. cibarius mushroom in the production of extracellular lipase under submerged fermentation, representing the first report on lipase production by this mushroom. Various physicochemical factors were optimized via one-factor-at-a time (OFAT) method. Maximum enzyme production was recorded when the mushroom mycelium was grown at 30 °C on pH 6.0 for 96 h in the medium supplemented with 1% [(v/v)] olive oil. Productivity of enzyme was affected by variation in the nitrogen sources, carbon sources, metal ions and NaCl salt. Glucose and peptone significantly enhanced enzyme production as carbon and nitrogen sources, respectively. Stimulatory and inhibitory effects were found by Ca 2+ and Zn 2+ ions, respectively. Furthermore, Box-Behnken Design (BBD) of Response Surface Methodol- ogy (RSM) was employed to optimize the interactive effects of specific media components like glucose, olive oil and CaCl 2 . The regression model was significant with a coefficient of determination (R2 ) value of 0.9483. Statistically optimized design (RSM) resulted approximately two-fold increase (23.5–42.283 UmL −1 ) of lipase production than classical optimization method (OFAT), confirmed the validation of model. The kinetic parameters for p-nitrophenyl palmitate hydrolysis, K m and V max were 5.24 mM and 0.768 mmol/min/mg respectively, established a high affinity for the substrate.

Item Type: Article
Uncontrolled Keywords: Lipase, Cantharellus cibarius, Submerged fermentation, Response surface methodology, Box-Behnken design
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: Beáta Bavalicsné Kerekes
Date Deposited: 29 Aug 2023 12:06
Last Modified: 29 Aug 2023 12:06
URI: http://real.mtak.hu/id/eprint/172254

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