Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion

Udyavara Nagaraj, Vignesh and Juhász, Tünde and Quemé Peña, Mayra and Szigyártó, Imola Csilla and Bogdán, Dóra and Wacha, András Ferenc and Mihály, Judith and Románszki, Loránd and Varga, Zoltán and Andréasson, Joakim and Mándity, István and Beke-Somfai, Tamás (2022) Stimuli-Responsive Membrane Anchor Peptide Nanofoils for Tunable Membrane Association and Lipid Bilayer Fusion. ACS APPLIED MATERIALS & INTERFACES, 14 (50). pp. 55320-55331. ISSN 1944-8244

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Self-assembled peptide nanostructures with stimuli-responsive features are promising as functional materials. Despite extensive research efforts, water-soluble supramolecular constructs that can interact with lipid membranes in a controllable way are still challenging to achieve. Here, we have employed a short membrane anchor protein motif (GLFD) and coupled it to a spiropyran photoswitch. Under physiological conditions, these conjugates assemble into ∼3.5 nm thick, foil-like peptide bilayer morphologies. Photoisomerization from the closed spiro (SP) form to the open merocyanine (MC) form of the photoswitch triggers rearrangements within the foils. This results in substantial changes in their membrane-binding properties, which also varies sensitively to lipid composition, ranging from reversible nanofoil reformation to stepwise membrane adsorption. The formed peptide layers in the assembly are also able to attach to various liposomes with different surface charges, enabling the fusion of their lipid bilayers. Here, SP-to-MC conversion can be used both to trigger and to modulate the liposome fusion efficiency.

Item Type: Article
Uncontrolled Keywords: self-assembly, liposomes, membrane activity, spiropyran, peptide bilayer, lipid bilayer fusion
Subjects: Q Science / természettudomány > QC Physics / fizika > QC173.4 Material science / anyagtudomány
Q Science / természettudomány > QD Chemistry / kémia
Depositing User: MTMT SWORD
Date Deposited: 05 Sep 2023 09:16
Last Modified: 05 Sep 2023 09:16

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