Hypertension Exacerbates Cerebrovascular Oxidative Stress Induced by Mild Traumatic Brain Injury: Protective Effects of the Mitochondria-Targeted Antioxidative Peptide SS-31

Czigler, András and Tóth, Luca and Szarka, Nikolett and Berta, Gergely and Amrein, Krisztina and Czeiter, Endre and Lendvai-Emmert, Dominika and Bodó, Kornélia and Koller, Ákos and Ungvári, Zoltán and Büki, András and Tóth, Péter (2019) Hypertension Exacerbates Cerebrovascular Oxidative Stress Induced by Mild Traumatic Brain Injury: Protective Effects of the Mitochondria-Targeted Antioxidative Peptide SS-31. JOURNAL OF NEUROTRAUMA, 36 (23). pp. 3309-3315. ISSN 0897-7151 (print); 1557-9042 (online)

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Abstract

Traumatic brain injury (TBI) induces cerebrovascular oxidative stress, which is associated with neurovascular uncoupling, autoregulatory dysfunction and persisting cognitive decline in both preclinical models and patients. However, single mild TBI, the most frequent form of brain trauma increases cerebral generation of reactive oxygen species (ROS) only transiently. We hypothesized, that co-morbid conditions may exacerbate long term ROS generation in cerebral arteries after mTBI. Since hypertension is the most important cerebrovascular risk factor in populations prone to mild brain trauma, we induced mTBI in normotensive and spontaneously hypertensive rats (SHR) and assessed changes in cytoplasmic and mitochondrial superoxide (O2-) production by confocal microscopy in isolated middle cerebral arteries (MCA) two weeks after mTBI using dihydroethidine (DHE) and the mitochondria-targeted redox sensitive fluorescent indicator dye MitoSox. We found that mTBI induced a significant increase in long term cytoplasmic and mitochondrial O2- production in MCAs of SHRs and increased expression of the NADPH oxidase subunit Nox4, which were reversed to the normal level by treating the animals with the cell-permeable, mitochondria-targeted antioxidant peptide SS-31(5.7 mg kg-1 day-1 , i.p.). Persistent mTBI-induced oxidative stress in MCAs of SHRs was significantly decreased by inhibiting vascular NADPH oxidase (apocyinin). We propose, that hypertension- and mTBI-induced cerebrovascular oxidative stress likely lead to persistent dysregulation of CBF and cognitive dysfunction, which might be reversed by SS-31 treatment.

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