Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats

AL Doghmi, Afnan and Barta, Bence Pál and Egyed-Kolumbán, Abigél and Onhausz, Benita and Kiss, Szilvia and Balázs, János and Szalai, Zita and Bagyánszki, Mária and Bódi, Nikolett (2023) Gut Region-Specific Interleukin 1β Induction in Different Myenteric Neuronal Subpopulations of Type 1 Diabetic Rats. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (6). No-5804. ISSN 1661-6596

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Interleukin 1β (IL1β) is a pro-inflammatory cytokine that may play a crucial role in enteric neuroinflammation in type 1 diabetes. Therefore, our goal is to evaluate the effects of chronic hyperglycemia and insulin treatment on IL1β immunoreactivity in myenteric neurons and their different subpopulations along the duodenum–ileum–colon axis. Fluorescent immunohistochemistry was used to count IL1β expressing neurons as well as the neuronal nitric oxide synthase (nNOS)- and calcitonin gene-related peptide (CGRP)-immunoreactive myenteric neurons within this group. Tissue IL1β level was measured by ELISA in muscle/myenteric plexus-containing homogenates. IL1β mRNA was detected by RNAscope in different intestinal layers. The proportion of IL1β-immunoreactive myenteric neurons was significantly higher in the colon than in the small intestine of controls. In diabetics, this proportion significantly increased in all gut segments, which was prevented by insulin treatment. The proportion of IL1β-nNOS-immunoreactive neurons only increased in the diabetic colon, while the proportion of IL1β-CGRP-immunoreactive neurons only increased in the diabetic ileum. Elevated IL1β levels were also confirmed in tissue homogenates. IL1β mRNA induction was detected in the myenteric ganglia, smooth muscle and intestinal mucosa of diabetics. These findings support that diabetes-related IL1β induction is specific for the different myenteric neuronal subpopulations, which may contribute to diabetic motility disturbances.

Item Type: Article
Uncontrolled Keywords: interleukin 1β enteric nervous system; myenteric neurons; neuronal nitric oxide synthase; calcitonin gene-related peptide; type 1 diabetes; hyperglycemia; insulin; gut regions
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QP Physiology / élettan
Depositing User: MTMT SWORD
Date Deposited: 13 Sep 2023 12:49
Last Modified: 13 Sep 2023 12:49

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