Application of Lacunarity for Quantification of Single Molecule Localization Microscopy Images

H. Kovács, Bálint Barna and Varga, Dániel and Sebők, Dániel and Majoros, Hajnalka and Polanek, Róbert and Pankotai, Tibor and Hideghéty, Katalin and Kukovecz, Ákos and Erdélyi, Miklós (2022) Application of Lacunarity for Quantification of Single Molecule Localization Microscopy Images. CELLS, 11 (19). ISSN 2073-4409


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The quantitative analysis of datasets achieved by single molecule localization microscopy is vital for studying the structure of subcellular organizations. Cluster analysis has emerged as a multi-faceted tool in the structural analysis of localization datasets. However, the results it produces greatly depend on the set parameters, and the process can be computationally intensive. Here we present a new approach for structural analysis using lacunarity. Unlike cluster analysis, lacunarity can be calculated quickly while providing definitive information about the structure of the localizations. Using simulated data, we demonstrate how lacunarity results can be interpreted. We use these interpretations to compare our lacunarity analysis with our previous cluster analysis-based results in the field of DNA repair, showing the new algorithm’s efficiency.

Item Type: Article
Additional Information: Department of Optics and Quantum Electronics, University of Szeged, Szeged, 6720, Hungary Department of Applied and Environmental Chemistry, University of Szeged, Szeged, 6720, Hungary Institute of Pathology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, 6725, Hungary Centre of Excellence for Interdisciplinary Research, Development and Innovation, University of Szeged, Szeged, 6723, Hungary Biomedical Applications Group, ELI-ALPS Research Institute, ELI-HU Non-Profit Ltd., Szeged, 6728, Hungary Department of Oncotherapy, University of Szeged, Szeged, 6720, Hungary Genome Integrity and DNA Repair Group, Hungarian Centre of Excellence for Molecular Medicine (HCEMM), University of Szeged, Szeged, 6728, Hungary Export Date: 31 March 2023 Correspondence Address: Kovács, B.B.H.; Department of Optics and Quantum Electronics, Hungary; email: Correspondence Address: Erdélyi, M.; Department of Optics and Quantum Electronics, Hungary; email: Funding details: 739593 Funding details: National Renewable Energy Laboratory, NREL, RRF-2.3.1-21-2022-00009 Funding details: European Commission, EC Funding details: Magyar Tudományos Akadémia, MTA, BO/27/20, UNKP-21-5-SZTE-563, UNKP-22-5-SZTE-318 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, TKP2021-NVA-19 Funding text 1: This research was partially supported by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-NVA funding scheme (no. TKP2021-NVA-19); the Bolyai János Research Scholarship of the Hungarian Academy of Sciences (BO/27/20); the UNKP-22-5-SZTE-318 and UNKP-21-5-SZTE-563 grants; the Recovery and Resilience Facility of the European Union within the framework of the Széchenyi Plan Plus programme (National Laboratory for Renewable Energy project, no. RRF-2.3.1-21-2022-00009). The project has received funding from the EU’s Horizon 2020 research and innovation program under grant agreement No. 739593.
Subjects: Q Science / természettudomány > QC Physics / fizika > QC04 Electricity. Magnetism. Electromagnetism / villamosság, mágnesesség, elektromágnessesség
Q Science / természettudomány > QD Chemistry / kémia > QD02 Physical chemistry / fizikai kémia
R Medicine / orvostudomány > RB Pathology / patológia, kórtan
R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0254 Neoplasms. Tumors. Oncology (including Cancer) / daganatok, tumorok, onkológia
R Medicine / orvostudomány > RZ Other systems of medicine / orvostudomány egyéb területei
Depositing User: MTMT SWORD
Date Deposited: 22 Sep 2023 10:48
Last Modified: 22 Sep 2023 10:48

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