Koplányi, Gábor and Bell, Evelin and Molnár, Zsófia Klára and Tóth, Gergő Dániel and Józó, Muriel and Szilágyi, András Ferenc and Ender, Ferenc and Pukánszky, Béla and Vértessy, Beáta (Grolmuszné) and Poppe, László and Balogh Weiser, Diána (2021) Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning. CATALYSTS, 11 (10). No. 1149. ISSN 2073-4344
|
Text
catalysts-2021.pdf Available under License Creative Commons Attribution. Download (13MB) | Preview |
Abstract
Immobilization of the recombinant, plant-derived Petroselinum crispum phenylalanine ammonia lyase (PcPAL) in electrospun matrices have the potential to create promising, easy-to-use biocatalysts. Polylactic acid (PLA) a biologically inert, commercial biopolymer, was chosen as the material of the carrier system. PLA could be electrospun properly only from water-immiscible organic solvents, which limits its application as a carrier of sensitive biological objects. The emulsion electrospinning is a proper solution to overcome this issue using non-ionic emulsifiers with different hydrophilic-lipophilic balance (HLB) values. The stabilized emulsion could protect the sensitive PcPAL dissolved in the aqueous buffer phase and improve fiber formation, plus help to keep the biocatalytic activity of enzymes. In this study, the first approach is described to produce PLA nanofibers containing PcPAL enzymes by emulsion electrospinning and to use the resulted biocatalyst in the ammonia elimination reaction from L-phenylalanine.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | electrospinning; recombinant enzyme; enzyme entrapment; emulsion electrospinning; phenylalanine ammonia-lyase |
Subjects: | Q Science / természettudomány > QD Chemistry / kémia Q Science / természettudomány > QD Chemistry / kémia > QD02 Physical chemistry / fizikai kémia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 25 Sep 2023 08:27 |
Last Modified: | 25 Sep 2023 08:27 |
URI: | http://real.mtak.hu/id/eprint/174579 |
Actions (login required)
![]() |
Edit Item |