González Morales, Nicanor and Marescal, Océane and Szikora, Szilárd and Katzemich, Anja and Correia-Mesquita, Tuana and Bíró, Péter and Erdélyi, Miklós and Mihály, József and Schöck, Frieder (2023) The oxoglutarate dehydrogenase complex is involved in myofibril growth and Z-disc assembly in Drosophila. JOURNAL OF CELL SCIENCE, 136 (13). No-jcs260717. ISSN 0021-9533
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Abstract
Myofibrils are long intracellular cables specific to muscles, composed mainly of actin and myosin filaments. The actin and myosin filaments are organized into repeated units called sarcomeres, which form the myofibrils. Muscle contraction is achieved by the simultaneous shortening of sarcomeres, which requires all sarcomeres to be the same size. Muscles have a variety of ways to ensure sarcomere homogeneity. We have previously shown that the controlled oligomerization of Zasp proteins sets the diameter of the myofibril. Here, we looked for Zasp-binding proteins at the Z-disc to identify additional proteins coordinating myofibril growth and assembly. We found that the E1 subunit of the oxoglutarate dehydrogenase complex localizes to both the Z-disc and the mitochondria, and is recruited to the Z-disc by Zasp52. The three subunits of the oxoglutarate dehydrogenase complex are required for myofibril formation. Using super-resolution microscopy, we revealed the overall organization of the complex at the Z-disc. Metabolomics identified an amino acid imbalance affecting protein synthesis as a possible cause of myofibril defects, which is supported by OGDH-dependent localization of ribosomes at the Z-disc.
Item Type: | Article |
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Uncontrolled Keywords: | Drosophila, Muscle, Myofibril, Ogdh, TCA cycle, Zasp |
Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 25 Sep 2023 11:35 |
Last Modified: | 25 Sep 2023 11:35 |
URI: | http://real.mtak.hu/id/eprint/174811 |
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