Four-in-One: HSQC, HSQC-TOCSY (or H2BC), TOCSY, and Enhanced HMBC Spectra Integrated into a Single NO Relaxation Delay (NORD) NMR Experiment

Farkas, László Bence and Timári, István and E Kövér, Katalin and Sørensen, Ole W. (2022) Four-in-One: HSQC, HSQC-TOCSY (or H2BC), TOCSY, and Enhanced HMBC Spectra Integrated into a Single NO Relaxation Delay (NORD) NMR Experiment. JOURNAL OF MAGNETIC RESONANCE, 343. No.-107297. ISSN 1090-7807 (print); 1096-0856 (online)

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Abstract

The NMR pulse sequence design strategy of NORD (NO Relaxation Delay) is extended to design of two new three-module experiments, NORD {HMBC}-{HSQC-TOCSY}-{TOCSY} and NORD {HMBC}-{2BOB}-{TOCSY}, each delivering four spectra - HMBC, HSQC, TOCSY, and either HSQC-TOCSY or H2BC. Compared to individual recording of these spectra particularly the sensitivity of the least sensitive mod-ule, HMBC, is enhanced by designing the homonuclear TOCSY module to allow buildup of magnetization pertinent to HMBC during its execution. Effectively, the sensitivity of the heteronuclear modules is boosted at the expense of the inherently much higher TOCSY sensitivity, thus resulting in a significant saving in spectrometer time.

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