Kaszler, Nikolett and Benkő, Péter and Molnár, Á. and Zámbori, A. and Fehér, Attila and Gémes, Katalin (2023) Absence of Arabidopsis Polyamine Oxidase 5 Influences the Cytokinin-Induced Shoot Meristem Formation from Lateral Root Primordia. PLANTS, 12 (3). ISSN 2223-7747
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Abstract
Lateral root primordia (LRPs) of Arabidopsis can be directly converted to shoot meristems (SMs) by the application of exogenous cytokinin. Here, we report that Arabidopsis POLYAMINE OXIDASE 5 (AtPAO5) contributes to this process, since the rate of SM formation from LRPs was significantly lower in the pao5-2 knockout mutant. Furthermore, the presented experiments showed that AtPAO5 influences SM formation via controlling the thermospermine (T-Spm) level. Gene expression analyses supported the view that the pao5-2 mutation as well as exogenous T-Spm downregulate the expression of the class 3 haemoglobin coding genes AtGLB1 and AtGLB2. AtGLB1 and 2 have been reported to augment cytokinin sensitivity, indirectly inhibiting the expression of type-A ARABIDOPSIS RESPONSE REGULATORs (ARRs). In agreement, the same ARR-coding genes were found to be upregulated in the pao5-2 mutant. Although GLB proteins might also control cytokinin-induced nitric oxide (NO) accumulation, we could not find experimental evidence for it. Rather, the negative effect of NO-donor treatment on AtPAO5 gene expression and SM formation was seen. Nevertheless, a hypothetical pathway is set up explaining how AtPAO5 may affect direct shoot meristem formation, controlling cytokinin sensitivity through T-Spm and GLBs. © 2023 by the authors.
Item Type: | Article |
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Uncontrolled Keywords: | nitric oxide; Arabidopsis thaliana; POLYAMINE OXIDASE; Haemoglobins; cytokinin sensitivity; direct shoot regeneration; |
Subjects: | Q Science / természettudomány > QK Botany / növénytan Q Science / természettudomány > QK Botany / növénytan > QK10 Plant physiology / növényélettan |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 26 Sep 2023 10:43 |
Last Modified: | 26 Sep 2023 10:43 |
URI: | http://real.mtak.hu/id/eprint/174968 |
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