Phenotypic and genotypic assessment of elite rice varieties for brown plant hopper (Nilaparvata lugens Stål.) resistance

Muduli, Lakesh and Dash, Manasi and Das Mohapatra, Shyamaranjan and Mohapatra, Kiran Kumar and Nayak, Hari Sankar and Bastia, Debendra Nath and Pradhan, Banshidhar and Tripathy, Swapan Kumar and Jena, Ram Chandra and Pradhan, Sukanta Kumar (2023) Phenotypic and genotypic assessment of elite rice varieties for brown plant hopper (Nilaparvata lugens Stål.) resistance. CEREAL RESEARCH COMMUNICATIONS, 51 (4). pp. 821-833. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Popular rice varieties from India which exhibited field resistance against Brown Plant Hopper attack were selected to validate their resistance reaction. Genotyping using twenty-four gene-specific markers for BPH resistance was carried out. Besides this, phenotyping of morpho-economic traits along with resistance reaction was performed. Stepwise regression of phenotypic data showed that the traits viz. trichome number, leaf area and leaf number per tiller were significantly correlated to BPH damage score, while the traits, grains per panicle, tiller number, plant height and leaf number per plant contributed significantly towards seed yield. It was observed that there was a positive association between leaf area and BPH damage while trichome number was negatively associated, causing decreasing value in damage score. In the case of yield, except leaf number per plant, others traits showed a positive association. Further, PCA biplot analysis results confirmed the findings. Phenotypically, the rice variety Hasanta exhibited the highest BPH resistance followed by Jyotirmayee, Mahanadi, Konark and Pratap. Out of the twenty-four gene-specific markers, six markers exhibited polymorphism and RM247 showed the highest polymorphic information content (PIC) value. Cluster analysis of the varieties based on the marker data placed the variety Hasanta in a separate cluster, confirming its distinctness. Genotyping confirmed the genetic nature of BPH resistance in Hasanta variety. The cluster 2.2a included the highest number of varieties based on genetic similarity although it comprised of resistant, moderately resistant and susceptible types. This finding indicated that phenotypic variation within genotypic similar groups might be due to environment or different sources of origin. Hence, this phenotypic and genotypic variation among the genotypes would be useful for selecting elite and potential BPH-resistant donors in a crop improvement program.

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