Karancsi, Zita and Kovács, Dóra and Csikó, György and Palócz, Orsolya and Jerzsele, Ákos and Gálfi, Péter and Farkas, Orsolya (2023) Effect of hydroxylated and methylated flavonoids on cytochrome P450 activity in porcine intestinal epithelial cells. ACTA VETERINARIA HUNGARICA, 71 (1). pp. 16-24. ISSN 0236-6290
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Abstract
Abstract Cytochrome P450 (CYP) oxidases are among the main metabolizing enzymes that are responsible for the transformation of xenobiotics, including clinically important drugs. Their activity can be influenced by several compounds leading to decreased efficacy or increased toxicity of co-administered medicines. Flavonoids exert various beneficial effects on human and animal health; therefore they are used as food and feed supplements. However, they are also well-known for their CYP modulating potential. Since the amount of CYP enzymes is highest in the liver, interaction studies are mainly conducted in hepatocytes, however, CYP activity in the gastrointestinal tract is also remarkable. In this study, effects of apigenin (API), quercetin (QUE) and their methylated derivatives trimethylapigenin (TM-API), 3-O-methylquercetin (3M-QUE) and 3′,7-di-O-methylquercetin (3′7DM-QUE) on the CYP enzyme activity was examined in IPEC-J2 porcine intestinal epithelial cells. Potential food-drug interactions were studied using flavonoid treatment in combination with inducer and inhibitor compounds. API, TM-API, QUE and 3M-QUE significantly inhibited the CYP3A29 enzyme, while 3′7DM-QUE did not alter its activity. Enzyme inhibition has also been observed in case of some food-drug combinations. Our results support previous findings about CYP modulating effects of flavonoids and highlights the possibility of interactions when flavonoid-containing supplements are consumed during drug treatments.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: European Union - European Social Fund [EFOP-3.6.3-VEKOP-16-2017-00005, TKP2020-NKA-01]; National Research, Development and Innovation Fund of Hungary [2020-4.1.1-TKP2020] Funding text: We are grateful to Dr. Jody Gookin for providing the IPEC-J2 cell line and to Lubov Nyikolajevna Bakumenko for contributing to the investigations. This research was funded by the European Union and co-financed by the European Social Fund (grant agreement no. EFOP-3.6.3-VEKOP-16-2017-00005, project title: "Strengthening the scienti fi c replacement by supporting the academic workshops and programs of students, developing a mentoring process ") . Funding was received from project no. TKP2020-NKA-01 that has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, fi nanced under the Temateruleti Kivalosagi Program 2020 (2020-4.1.1-TKP2020) funding scheme. |
| Subjects: | S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 15 Feb 2024 06:55 |
| Last Modified: | 15 Feb 2024 06:55 |
| URI: | https://real.mtak.hu/id/eprint/188356 |
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