Budavári, Bálint and Karancsi, Áron and Pinke, Balázs Gábor and Pállinger, Éva and Juriga-Tóth, Krisztina and Király, Márton and Szász, Zsófia and Voszka, István and Molnár, Kolos and Kőhidai, László and Jedlovszky-Hajdu, Angela and S. Nagy, Krisztina (2024) Long-term shelf-life liposomes for delivery of prednisolone and budesonide. JOURNAL OF MOLECULAR LIQUIDS, 394. No-123756. ISSN 0167-7322
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Abstract
Liposomes are nanoscale drug delivery systems built up from lipid layers and are able to spontaneously self- assemble in an aqueous environment. Both hydrophilic and hydrophobic drugs can be delivered by liposomes and this kind of nanoformulation offers many advantages regarding biodistribution, drug absorption and controlled drug release. Corticosteroids as lipophilic molecules are able to integrate into the lipid bilayer. This novel approach can improve the efficacy of several anti-inflammatory, such as asthma therapy. Our aim was to create liposomes with long shelf-life, which can incorporate and release corticosteroids such as Prednisolone (Pred) and Budesonide (Bud) at the temperature of inflamed tissues. Two kinds of liposome samples were prepared from three different kinds of phospholipids to get unilamellar vesicles with 100 nm in diameter and characterize their physicochemical properties and effect on living cells. Their main phase transition tem- perature in the physiologically relevant temperature range was measured by differential scanning calorimetry. According to the size distributions determined by dynamic light scattering, all drug-containing liposomes were stable for 6 months. All of the liposome types have a slightly negative zeta potential value. The Fourier-transform infrared spectroscopy revealed no chemical interaction between the drug and lipid molecules. The entrapment efficacy was determined by size-exclusion gel chromatography combined with UV–VIS spectrophotometry and it was very high in both cases (between 70 and 87%). The drug leakage was 35–40% for Pred and 6–8% for Bud in the first 30 min. The effect of liposomal drugs on cell viability was measured on the EBC-1 human lung carcinoma cell line. Neither the free corticosteroids nor their liposomal form were toxic to the cells. The cellular inter- nalization of the liposomes was proved by flow cytometry and confocal microscopy. In summary, these liposomes could be useful in the delivery of corticosteroids (Pred or particularly Bud) in more effective asthma therapy, having fewer side effects due to the nanoformulation.
Item Type: | Article |
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Uncontrolled Keywords: | Liposome, Prednisolone, Budesonide, Drug delivery, Asthma, Inhalation |
Subjects: | T Technology / alkalmazott, műszaki tudományok > TJ Mechanical engineering and machinery / gépészmérnöki tudományok |
Depositing User: | Dr. Tamás Tábi |
Date Deposited: | 16 Feb 2024 08:29 |
Last Modified: | 16 Feb 2024 08:29 |
URI: | https://real.mtak.hu/id/eprint/188436 |
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