Török, Dóra and Somoskői, Bence and Bordás, Lilla and Reglődi, Dóra and Cseh, Sándor (2023) Effect of pituitary adenylate cyclase-activating polypeptide supplementation, applied during or after vitrification on mouse embryo. ACTA VETERINARIA HUNGARICA, 71 (2). pp. 112-118. ISSN 0236-6290
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Abstract
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence and diverse functions. It occurs in high levels in the gonads suggesting a potential central role in reproduction. The aim of our study was to assess the effect of PACAP treatment during embryo vitrification on the developmental rate and the expression of the heparin-binding EGF-like growth factor gene (Hbegf). Mouse embryos, obtained from superovulated females were allocated into the four treatment groups. In EM1 and EM2, the embryos were prepared for vitrification in an Equilibration Solution that was supplemented with 1 or 2 μM PACAP1-38, respectively. The embyos in groups CM1 and CM2 were not treated prior to vitrification but were cultured in a medium supplemented with 1 or 2 μM PACAP1-38 after thawing. The Vitrified Control group consisted of embryos vitrified and thawed then cultured without PACAP1-38 treatment. A non-vitrified, non-treated Fresh Control group was also used. After 24 h of culture, the developmental rate of the embryos, as well as the relative expression level of the Hbegf gene, as determined by qPCR, were compared among groups. Higher developmental rate and Hbegf gene expression level were found in the embryos treated with a higher concentration of PACAP. These results indicate that PACAP treatment has a beneficial effect on the survival and development of vitrified/thawed mouse embryos.
Item Type: | Article |
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Additional Information: | Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [2020-4.1.1-TKP2020, 134887]; Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary [FK_20] Funding text: The project was supported by the Hungarian Research Fund (OTKA 115874); NKFIHK135457; TKP2020-IKA-08,MTA-TKI-14016. Project no. TKP2020-NKA-01 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the Tematerueleti Kivalosagi Program 2020 (2020-4.1.1-TKP2020) funding scheme. Project no. 134887 has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the FK_20 funding scheme. Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary,within the framework of the EGA-16 project of the University Pecs.r financed under the Temateruleti Kivalosagi Program 2020 (2020-4.1.1-TKP2020) funding scheme. Project no. 134887 has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the FK_20 funding scheme. Thematic Excellence Program 2021 Health Sub-programme of the Ministry for Innovation and Technology in Hungary, within the framework of the EGA-16 project of the Uni-versity Pecs. |
Uncontrolled Keywords: | PACAP; embryo; Vitrification; cryoprotectant; HB-EGF; |
Subjects: | S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány |
SWORD Depositor: | MTMT SWORD |
Depositing User: | MTMT SWORD |
Date Deposited: | 23 Feb 2024 07:29 |
Last Modified: | 23 Feb 2024 07:29 |
URI: | https://real.mtak.hu/id/eprint/188820 |
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