REAL

Proline cis/trans Isomerization in Intrinsically Disordered Proteins and Peptides

Sebák, Fanni and Szolomájer, János and Papp, Nándor and Tóth, Gábor and Bodor, Andrea (2023) Proline cis/trans Isomerization in Intrinsically Disordered Proteins and Peptides. FRONTIERS IN BIOSCIENCE-LANDMARK, 28 (6). ISSN 2768-6701

[img] Text
pdf
Available under License Creative Commons Attribution.

Download (4MB)

Abstract

Background: Intrinsically disordered proteins and protein regions (IDPs/IDRs) are important in diverse biological processes. Lacking a stable secondary structure, they display an ensemble of conformations. One factor contributing to this conformational heterogeneity is the proline cis/trans isomerization. The knowledge and value of a given cis/trans proline ratio are paramount, as the different conformational states can be responsible for different biological functions. Nuclear Magnetic Resonance (NMR) spectroscopy is the only method to characterize the two co-existing isomers on an atomic level, and only a few works report on these data. Methods: After collecting the available experimental literature findings, we conducted a statistical analysis regarding the influence of the neighboring amino acid types (i ± 4 regions) on forming a cis-Pro isomer. Based on this, several regularities were formulated. NMR spectroscopy was then used to define the cis-Pro content on model peptides and desired point mutations. Results: Analysis of NMR spectra prove the dependence of the cis-Pro content on the type of the neighboring amino acid—with special attention on aromatic and positively charged sidechains. Conclusions: Our results may benefit the design of protein regions with a given cis-Pro content, and contribute to a better understanding of the roles and functions of IDPs.

Item Type: Article
Uncontrolled Keywords: intrinsically disordered proteins; NMR spectroscopy; proline; cis/trans isomerization; statistical analysis
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 18 Mar 2024 12:06
Last Modified: 18 Mar 2024 12:06
URI: https://real.mtak.hu/id/eprint/190514

Actions (login required)

Edit Item Edit Item