Vairimorpha (Nosema) ceranae előfordulása Magyarországon, mézelő méhek (Apis mellifera) bélmikrobiom-felmérése alapján = Vairimorpha (Nosema) ceranae distribution based on a honey bee gut microbiome survey in Hungary

Papp, Márton and Békési, László and Farkas, Róbert and Makrai, László and Maróti, Gergely and Solymosi, Norbert (2024) Vairimorpha (Nosema) ceranae előfordulása Magyarországon, mézelő méhek (Apis mellifera) bélmikrobiom-felmérése alapján = Vairimorpha (Nosema) ceranae distribution based on a honey bee gut microbiome survey in Hungary. MAGYAR ÁLLATORVOSOK LAPJA, 146 (3). pp. 149-159. ISSN 0025-004X

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Abstract

Az európai mézelő méh (Apis mellifera), világszerte jelentős rovarfaj a mezőgazdaság számára. A méhcsaládokat számos kórokozó fenyegeti, köztük a Vairimorpha (Nosema) apis és Vairimorpha (Nosema) ceranae egysejtű gomba. Sokáig csupán a V. apis okozta kórkép (nosemosis apium – gyomorvész) volt ismert, azonban a közelmúltban egyre inkább az újonnan behurcolt V. ceranae előfordulása és kórtani szerepe vált jelentőssé. Egy korábbi vizsgálatukban a szerzők klimatikusan reprezentatív mintavételt végeztek a hazai méhcsaládokból márciusban és májusban, a méhek bélmikrobiomjának shotgun metagenomikai vizsgálata céljából. Minden mintában azonosítottak V. ceranae eredetű szekvenciákat. A kórokozó szignifikánsan gyakoribb volt májusban, valamint ugyanebben a mintavételi időpontban a csapadékosabb területeken. A V. apis fajnak nem találták a nyomát. | Background: Apis mellifera is one of the most important arthropod species for agriculture. Several factors are known to compromise their health, posing a serious economic risk. Among these factors, pathogens and mainly parasites are especially important, including the single-celled fungi Vairimorpha apis and Vairimorpha ceranae. Historically, V. ceranae was not considered to infect A. mellifera; however, recently, it was found in this species as well and has become more prominent worldwide. Objective: During a shotgun metagenomic survey on honey bees’ gut bacteriome, we found sequences originating from V. ceranae in all of our samples. Our findings of this species in the Hungarian A. mellifera gut metagenome is presented in this study. Materials and Methods: We have performed a climatically representative sampling on the Hungarian honey bee population in 2019. Twenty apiaries were selected for the analysis and sampled twice in the honey-producing season, at its onset (March) and peak (May). Shotgun metagenomic sequencing was performed to determine the microbiome composition of the samples. We have statistically analyzed the differences in V. ceranae relative abundance between the two sampling times and determined the effect of temperature and precipitation. Results and Discussion: Reads classified as V. ceranae were found in all twenty samples at both sampling times, which indicates that this species is widely distributed in Hungary. Interestingly, V. ceranae was significantly more abundant in May, which could support the observations that the epidemiology of this species indeed shows a seasonal pattern. Despite the previous importance of V. apis, we were unable to identify this species in our samples. Considering the environmental factors, V. ceranae was significantly more abundant in samples from regions with higher precipitation in May.

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